3GS5
An all-RNA hairpin ribozyme A38N1dA variant with a product mimic substrate strand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-09-08 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.830, 93.830, 132.090 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.850 - 2.750 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.24300 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.910 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.488 | 0.488 |
Number of reflections | 9256 | |
<I/σ(I)> | 24.4 | 2.3 |
Redundancy | 4.75 | 4.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20.5% (w/v) PEG 2K MME, 0.10 M Na-Cacodylate, 0.25 M Li2SO4, 2.5 mM Co(NH3)6Cl3, 2 mM spermidine-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 2K MME | ||
10 | 1 | 2 | spermidine-HCl | ||
2 | 1 | 1 | Na-Cacodylate | ||
3 | 1 | 1 | Li2SO4 | ||
4 | 1 | 1 | Co(NH3)6Cl3 | ||
5 | 1 | 1 | spermidine-HCl | ||
6 | 1 | 2 | PEG 2K MME | ||
7 | 1 | 2 | Na-Cacodylate | ||
8 | 1 | 2 | Li2SO4 | ||
9 | 1 | 2 | Co(NH3)6Cl3 |