3GRF
X-ray Structure of Ornithine Transcarbamoylase from Giardia lamblia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-23 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 63 |
| Unit cell lengths | 95.490, 95.490, 56.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.197 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a1s |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.970 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.306 |
| Number of reflections | 19952 | |
| Completeness [%] | 99.1 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 30% Polyethylene Glycol Monomethyl Ether 550, 0.1 M NaCl, 0.1 M Bicine buffer , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
