3GR8
Structure of OYE from Geobacillus kaustophilus, orthorhombic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-11-15 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.8148 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 82.394, 151.930, 143.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.214 - 2.500 |
Rwork | 0.188 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z41 |
RMSD bond length | 0.002 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 2.630 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 30975 | |
<I/σ(I)> | 4.4 | 1.3 |
Completeness [%] | 97.6 | 83.7 |
Redundancy | 8.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 50mM Bis-Tris, 50mM ammonium sulfate, 30% v/v pentaerythritole ethoxylate, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |