3GR7
Structure of OYE from Geobacillus kaustophilus, hexagonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-12-17 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54178 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 101.990, 101.990, 260.970 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.488 - 2.300 |
Rwork | 0.200 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z41 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.876 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 35123 | |
<I/σ(I)> | 18.7 | 4.8 |
Completeness [%] | 98.0 | 96.6 |
Redundancy | 15.8 | 15.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 50mM Bis-Tris, 50mM ammonium sulfate, 30% v/v pentaerythritole ethoxylate, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |