3GQU
Pyrococcus Horikoshii NOP5 RNA Binding Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93.15 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.502 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.604, 73.604, 114.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.683 - 2.500 |
| R-factor | 0.302 |
| Rwork | 0.299 |
| R-free | 0.33400 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.597 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.6) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.682 | 2.060 |
| High resolution limit [Å] | 1.957 | 1.950 |
| Rmerge | 0.040 | |
| Total number of observations | 5530 | |
| Number of reflections | 13956 | |
| <I/σ(I)> | 4.944 | 0.2 |
| Completeness [%] | 84.7 | 27 |
| Redundancy | 21.8 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 290 | 500MM POTASSIUM IODIDE, 2% PEG 3350, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
