3GQQ
Crystal structure of the human retinal protein 4 (unc-119 homolog A). Northeast Structural Genomics Consortium target HR3066a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.888, 79.560, 189.717 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.510 - 1.945 |
R-factor | 0.1924 |
Rwork | 0.191 |
R-free | 0.21380 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.262 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX (D/E) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.945 | 1.945 |
Rmerge | 0.088 | 0.537 |
Number of reflections | 166290 | |
<I/σ(I)> | 26.4 | 2.87 |
Completeness [%] | 99.6 | 100 |
Redundancy | 7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH UNDER OIL | 5 | 277 | 40% PEG 4000, 0.1M K acetate, 0.1M Na acetate, pH 5.0, MICROBATCH UNDER OIL, temperature 277K |