3GQJ
Crystal structure of Cell Inhibiting Factor (Cif) from Photorhabdus luminescens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.972 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 59.130, 59.130, 293.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.580 - 1.850 |
R-factor | 0.22819 |
Rwork | 0.226 |
R-free | 0.27360 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.254 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.960 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 26540 | |
<I/σ(I)> | 19.3 | 4.4 |
Completeness [%] | 97.2 | 88.1 |
Redundancy | 12 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 291 | 100 mM tri-Sodium Citrate pH 6.2, 2.45 M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |