3GQB
Crystal Structure of the A3B3 complex from V-ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-20 |
| Detector | ADSC QUANTUM Q215r |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 199.368, 199.368, 179.005 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.950 - 2.800 |
| R-factor | 0.25122 |
| Rwork | 0.250 |
| R-free | 0.28105 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.407 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.871 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 93409 | |
| Completeness [%] | 95.6 | 82.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | PEG8000, MPD, MgCl2, Tris-HCl, pH 7.5, VAPOR DIFFUSION, temperature 293K |
