3GQ9
Crystal Structure of the Bacteriophage phi29 gene product 12 N-terminal fragment in an apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-21 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.99 |
Spacegroup name | H 3 2 |
Unit cell lengths | 89.633, 89.633, 585.719 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.690 - 2.000 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.20400 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.262 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.690 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.079 | 0.039 | 0.397 |
Number of reflections | 61778 | ||
<I/σ(I)> | 24.302 | ||
Completeness [%] | 99.4 | 99.5 | 98.7 |
Redundancy | 7 | 7 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 298 | 100mM CHES at ~pH 10.0 and 8% PEG3K. Crystalls obtained were then soaked in a mother liquid containing 10mM EDTA for ~5 hours., VAPOR DIFFUSION, HANGING DROP, temperature 298K |