3GQ2

Crystal Structure of the Dimer of the p115 Tether Globular Head Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	77
Collection date	2005-12-08
Wavelength(s)	1.100
Spacegroup name	P 1 21 1
Unit cell lengths	78.164, 159.817, 81.447
Unit cell angles	90.00, 115.13, 90.00

Refinement procedure

Resolution	20.000 - 2.180
R-factor	0.205
Rwork	0.199
R-free	0.25400
RMSD bond length	0.012
RMSD bond angle	1.356
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.180	2.180
Rmerge		0.593
Number of reflections	91304
<I/σ(I)>	14.3
Completeness [%]	96.7	76.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	293	10% MPD, 18% PEG 4000, 0.1M Tris-HCL, pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K