3GPP
MutM encountering an intrahelical 8-oxoguanine (oxoG) lesion in EC3-T224P complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-03 |
| Detector | ADSC |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.243, 93.841, 103.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.070 - 2.150 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23500 |
| Starting model (for MR) | 1r2y protein part only |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.374 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
| Rmerge | 0.080 | 0.064 | 0.553 |
| Number of reflections | 24531 | ||
| <I/σ(I)> | 17.988 | ||
| Completeness [%] | 99.9 | 98.9 | 99.9 |
| Redundancy | 7 | 8.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8K, sodium cacodylate, glycerol, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8K | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 2 | PEG 8K | ||
| 5 | 1 | 2 | sodium cacodylate | ||
| 6 | 1 | 2 | glycerol |
