3GP2
Calmodulin bound to peptide from calmodulin kinase II (CaMKII)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.599, 38.409, 75.012 |
| Unit cell angles | 90.00, 125.27, 90.00 |
Refinement procedure
| Resolution | 25.080 - 1.460 |
| R-factor | 0.16138 |
| Rwork | 0.160 |
| R-free | 0.19218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.851 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.080 | 1.530 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.048 | 0.342 |
| Number of reflections | 28224 | |
| <I/σ(I)> | 13 | 3.5 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 4.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1 M sodium acetate pH 4.6, 0.2 M ammonium acetate, 30% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
