3GOM
Barium bound to the Holliday junction sequence d(TCGGCGCCGA)4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | OXFORD SAPPHIRE CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.200, 23.800, 37.410 |
| Unit cell angles | 90.00, 110.01, 90.00 |
Refinement procedure
| Resolution | 19.956 - 2.300 |
| R-factor | 0.264 |
| Rwork | 0.260 |
| R-free | 0.34900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nvy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.822 |
| Data reduction software | CrysalisPro (Red) |
| Data scaling software | CrysalisPro (Red) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.956 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.052 | 0.210 |
| Number of reflections | 2351 | |
| <I/σ(I)> | 3.21 | |
| Completeness [%] | 93.2 | 93.22 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | BaCl2, MPD, NaCacodylate , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | BaCl2 | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | Na Cacodylate | ||
| 4 | 1 | 2 | BaCl2 | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | Na Cacodylate |
