3GOF
Calmodulin bound to peptide from macrophage nitric oxide synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 180 |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.923, 57.773, 60.231 |
Unit cell angles | 90.00, 93.06, 90.00 |
Refinement procedure
Resolution | 9.340 - 1.450 |
R-factor | 0.14802 |
Rwork | 0.145 |
R-free | 0.20822 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.565 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 1.520 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 52623 | |
<I/σ(I)> | 4 | |
Completeness [%] | 97.0 | 95.4 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.6, 20% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |