3GOE
Molecular Mimicry of SUMO promotes DNA repair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-10 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.818 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 81.519, 81.519, 81.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 0.970 |
| Rwork | 0.145 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uyz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.000 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rmerge | 0.035 | 0.414 |
| Number of reflections | 50503 | |
| <I/σ(I)> | 30 | 2.8 |
| Completeness [%] | 95.0 | 100 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.75 | 293 | 0.2M Ca acetate, 100mM HEPES, 24% (w/v) PEG 4000, pH 7.75, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
