3GO7
Crystal Structure of M. tuberculosis ribokinase (Rv2436) in complex with ribose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.025, 119.207, 89.768 |
Unit cell angles | 90.00, 115.00, 90.00 |
Refinement procedure
Resolution | 42.220 - 2.500 |
R-factor | 0.179 |
Rwork | 0.176 |
R-free | 0.23700 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.399 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.063 | 0.019 | 0.438 |
Number of reflections | 26190 | ||
<I/σ(I)> | 19.959 | ||
Completeness [%] | 99.1 | 99.6 | 89.6 |
Redundancy | 3.6 | 3.7 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 295 | 0.2 M Sodium/potassium phosphate, 0.1 M Bis-Tris propane, 16% PEG 3350, pH 6.5, vapor diffusion, temperature 295K |