3GO2
Crystal structure of putative L-alanine-DL-glutamate epimerase from Burkholderia xenovorans strain LB400 bound to magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 104.697, 104.697, 145.828 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.153 |
| Rwork | 0.152 |
| R-free | 0.18500 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.333 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.126 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.251 |
| Number of reflections | 44736 | |
| <I/σ(I)> | 26 | 6.8 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 9 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.8 | 294 | 100mM Sodium acetate pH 4.8, 2.4M Sodium formate, VAPOR DIFFUSION, temperature 294K |
