3GNZ
Toxin fold for microbial attack and plant defense
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0052 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 69.630, 140.900, 49.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.400 - 1.350 |
R-factor | 0.15694 |
Rwork | 0.156 |
R-free | 0.18363 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gnu |
RMSD bond length | 0.008 |
RMSD bond angle | 1.218 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.450 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.050 | 0.281 |
Number of reflections | 53077 | |
<I/σ(I)> | 4.2 | |
Completeness [%] | 98.3 | 92.1 |
Redundancy | 3.8 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1M Tris HCl pH 9.0, 20% PEG 4000, 0.5M MgCl2, 5% methanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |