3GNE

Crystal structure of alginate lyase vAL-1 from Chlorella virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-12
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.7
Spacegroup name	P 1 21 1
Unit cell lengths	60.699, 72.026, 60.711
Unit cell angles	90.00, 112.35, 90.00

Refinement procedure

Resolution	10.000 - 1.200
R-factor	0.1313
R-free	0.17940
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	0.029
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.240
High resolution limit [Å]	1.200	1.200
Total number of observations	747443
Number of reflections	142201
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	15% PEG 4000, 15% iso-Propanol, 0.1M sodium citrate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K