3GND
Crystal Structure of E. coli LsrF in complex with Ribulose-5-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-18 |
| Detector | ADSC |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.741, 107.102, 169.516 |
| Unit cell angles | 90.00, 102.62, 90.00 |
Refinement procedure
| Resolution | 49.030 - 2.900 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gkf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.178 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.030 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.090 | 0.038 | 0.436 |
| Number of reflections | 111445 | ||
| <I/σ(I)> | 8.666 | ||
| Completeness [%] | 93.0 | 98.5 | 82.1 |
| Redundancy | 1.7 | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 4% PEG 400, 100 mM MgCl2, 2.3 M Ammonium Sulfate, pH 7.5, vapor diffusion, temperature 298K |
