3GN9
Crystal structure of the major pseudopilin from the type 2 secretion system of Vibrio vulnificus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-03 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.54180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.180, 104.510, 55.410 |
| Unit cell angles | 90.00, 106.16, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.860 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fu1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.249 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.900 | 38.900 | 1.910 |
| High resolution limit [Å] | 1.860 | 8.320 | 1.860 |
| Rmerge | 0.029 | 0.016 | 0.486 |
| Number of reflections | 78730 | 893 | 5544 |
| <I/σ(I)> | 43.1 | 1.7 | |
| Completeness [%] | 86.7 | 87.2 | 81.5 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 1.95M DL-Malic acid pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
