3GN3
Crystal structure of a putative protein-disulfide isomerase from Pseudomonas syringae to 2.5A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-13 |
| Detector | ADSC QUANTUM Q215r |
| Wavelength(s) | 0.97974 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 151.968, 151.968, 115.894 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.750 - 2.500 |
| R-factor | 0.20728 |
| Rwork | 0.206 |
| R-free | 0.23692 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.352 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.142 | 0.746 |
| Number of reflections | 27721 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M Hepes pH 7.5, 2M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
