3GMY
Crystal Structure of Beta-Lactamse Inhibitory Protein-Like Protein (BLP), Selenomethionine Derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-27 |
Detector | ADSC QUANTUM Q315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 101.167, 67.195, 41.208 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.000 - 1.700 |
R-factor | 0.20328 |
Rwork | 0.200 |
R-free | 0.27300 |
Structure solution method | SAD |
RMSD bond length | 0.027 |
RMSD bond angle | 2.090 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.990 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 30474 | |
<I/σ(I)> | 34.3 | |
Completeness [%] | 96.5 | 96.2 |
Redundancy | 7.6 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 8.5 | 294 | 11 mg/ml protein in 30 mM tris, 150 mM NaCl mixed 1:1 with well solution 30% PEG 4000, 0.2 M sodium acetate trihydrate, pH 8.5, hanging drop, temperature 294K |