3GMX
Crystal Structure of Beta-Lactamse Inhibitory Protein-Like Protein (BLP) at 1.05 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-28 |
Detector | ADSC QUANTUM Q315r |
Wavelength(s) | 1.11588 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.771, 99.925, 41.643 |
Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
Resolution | 49.900 - 1.050 |
R-factor | 0.126 |
Rwork | 0.125 |
R-free | 0.15400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.533 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.940 | 1.077 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.265 | |
Number of reflections | 114297 | |
<I/σ(I)> | 6.5 | 2.3 |
Completeness [%] | 86.1 | 65.9 |
Redundancy | 9.9 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under oil | 6.5 | 294 | starting concentration as follows: 5.5 mg/ml protein, 10% PEG 8000, 0.1 M magnesium acetate tetrahydrate, 50 mM sodium cacodylate, 75 mM NaCl, 15 mM tris, pH 6.5, microbatch under oil, temperature 294K |