3GMV
Crystal Structure of Beta-Lactamse Inhibitory Protein-I (BLIP-I) in Apo Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-13 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.735, 44.071, 45.291 |
Unit cell angles | 90.00, 104.58, 90.00 |
Refinement procedure
Resolution | 20.490 - 1.800 |
R-factor | 0.1874 |
Rwork | 0.185 |
R-free | 0.22695 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.350 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 17522 | |
<I/σ(I)> | 28.336 | 11.6 |
Completeness [%] | 91.0 | 68.7 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop vapor batch | 294 | 20 mg/ml protein in 50 mM tris, pH 8.0, 50 mM NaCl mixed 1:1 with well solution 30% PEG 4000, 0.3 M lithium chloride, 0.1 M TrisCl, pH 8.5, hanging drop vapor batch, temperature 294K |