3GMU

Crystal Structure of Beta-Lactamse Inhibitory Protein (BLIP) in Apo Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1992-12-17
Detector	SDMS TWIN AREA DETECTOR SYSTEM
Wavelength(s)	1.54056
Spacegroup name	C 1 2 1
Unit cell lengths	129.650, 26.190, 48.070
Unit cell angles	90.00, 113.82, 90.00

Refinement procedure

Resolution	59.300 - 1.980
R-factor	0.167
Rwork	0.164
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.841
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.340	2.030
High resolution limit [Å]	1.980	1.980
Number of reflections	9038
<I/σ(I)>	7.4	1.8
Completeness [%]	84.6	64.8
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	298	10 mg/ml protein, 20% saturated ammonium sulfate, 50 mM sodium citrate, pH 5.5, vapor diffusion, temperature 298K