3GMI
Crystal structure of a protein of unknown function from Methanocaldococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-17 |
Detector | SBC-2 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.935, 73.988, 85.024 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.810 - 1.910 |
R-factor | 0.1756 |
Rwork | 0.174 |
R-free | 0.20858 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.643 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.810 | 1.960 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.116 | 0.660 |
Number of reflections | 33426 | |
<I/σ(I)> | 17.3 | 1.27 |
Completeness [%] | 99.1 | 89.71 |
Redundancy | 8.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M Lithium chloride, 0.05M Magnesium sulfate, 8% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |