3GMG
Crystal structure of an uncharacterized conserved protein from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.815, 65.173, 85.013 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.000 - 1.500 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21100 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.390 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.723 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.081 | 0.289 |
Number of reflections | 50353 | |
<I/σ(I)> | 25.2 | 7.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.6 | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM Bis-Tris pH 6.5, 25% PEG 3350, 200mM Sodium chloride, VAPOR DIFFUSION, temperature 294K |