3GM3

Crystal Structure of the Focal Adhesion Targeting (FAT) Domain of Pyk2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 2 21
Unit cell lengths	28.462, 42.791, 129.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.380 - 2.600
R-factor	0.25625
Rwork	0.255
R-free	0.28871
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gm2
RMSD bond length	0.010
RMSD bond angle	1.216
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.4.0066)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	5467
Completeness [%]	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20% PEG 8000, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K