3GLV
Crystal structure of the lipopolysaccharide core biosynthesis protein from Thermoplasma volcanium GSS1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794, 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.391, 67.187, 99.032 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.640 - 1.990 |
R-factor | 0.19694 |
Rwork | 0.195 |
R-free | 0.24228 |
Structure solution method | MAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.733 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.640 | 2.040 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.107 | 0.644 |
Number of reflections | 26291 | |
<I/σ(I)> | 29.67 | 2.3 |
Completeness [%] | 94.9 | 69.61 |
Redundancy | 8.5 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M Calcium chloride, 0.1M Tris-HCl, 25% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |