3GLD
Crystal Structure of the Major Pilin from Streptococcus pyogenes E117A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-05-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.307, 52.149, 127.316 |
Unit cell angles | 90.00, 98.83, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.030 |
R-factor | 0.21487 |
Rwork | 0.212 |
R-free | 0.26471 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b2m |
RMSD bond length | 0.011 |
RMSD bond angle | 1.303 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 54403 | |
<I/σ(I)> | 32.6 | 5.8 |
Completeness [%] | 95.8 | 84.9 |
Redundancy | 5.9 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.2M Potassium acetate, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K |