3GLA
Crystal Structure of the hspA from Xanthomonas axonopodis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.43 |
| Spacegroup name | H 3 |
| Unit cell lengths | 128.674, 128.674, 55.251 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.970 - 1.640 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gme |
| RMSD bond length | 0.037 |
| RMSD bond angle | 3.221 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.973 | 1.710 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 41715 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1 M Tris HCl, 1.2 M (NH4)2HPO4, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
