3GL5
Crystal structure of probable DsbA oxidoreductase SCO1869 from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.360, 60.360, 132.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.24632 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.068 | 0.451 |
| Number of reflections | 14072 | |
| <I/σ(I)> | 54.5 | 7.58 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.6 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | 0.1M Bis-tris pH 6.5, 0.2M Na Acetate, 30% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
