3GL3
Crystal structure of a putative Thiol:disulfide interchange protein DsbE from Chlorobium tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.197, 42.067, 82.522 |
| Unit cell angles | 90.00, 108.77, 90.00 |
Refinement procedure
| Resolution | 39.070 - 2.090 |
| R-factor | 0.272 |
| Rwork | 0.263 |
| R-free | 0.29600 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.070 | 2.220 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.091 | 0.230 |
| Number of reflections | 29179 | |
| <I/σ(I)> | 9.2 | 2 |
| Completeness [%] | 95.1 | 100 |
| Redundancy | 6.8 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.4M Sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
