3GKZ
Crystal structures of a therapeutic single chain antibody in complex methamphetamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.338, 65.265, 48.513 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 38.640 - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.640 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| <I/σ(I)> | 33.2 | 4.9 |
| Completeness [%] | 98.6 | 89.8 |
| Redundancy | 6.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 287 | 1.15M sidium citrate, 0.28M imidazole malate, pH 8.0, VAPOR DIFFUSION, temperature 287K |
