3GKY
The Structural Basis of an ER Stress-Associated Bottleneck in a Protein Folding Landscape
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-11 |
Wavelength(s) | 0.948 |
Spacegroup name | H 3 |
Unit cell lengths | 78.225, 78.225, 36.712 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.580 - 1.800 |
Rwork | 0.209 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1trz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.580 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.182 |
Number of reflections | 7299 | |
<I/σ(I)> | 16.5 | 7.4 |
Completeness [%] | 94.3 | 90.1 |
Redundancy | 6.4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 0.02 M Tris, 0.05 M sodium citrate, 5% acetone, 0.03% phenol, 0.01% zinc acetate, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |