3GKU
Crystal structure of a probable RNA-binding protein from Clostridium symbiosum ATCC 14940
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97926, 0.97937 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 126.992, 126.992, 106.185 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.720 - 2.950 |
R-factor | 0.22316 |
Rwork | 0.220 |
R-free | 0.28741 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.843 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 | 3.000 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.135 | 0.758 |
Number of reflections | 21140 | |
<I/σ(I)> | 13.5 | 1.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 10% w/v Isopropanol, 0.1M HEPES, 20% w/v PEG 4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |