3GKQ
Terminal oxygenase of carbazole 1,9a-dioxygenase from Novosphingobium sp. KA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-11-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.147, 159.019, 167.774 |
| Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
| Resolution | 47.070 - 2.100 |
| R-factor | 0.2144 |
| Rwork | 0.201 |
| R-free | 0.43600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.088 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.074 |
| Number of reflections | 355392 |
| <I/σ(I)> | 3.5 |
| Completeness [%] | 99.9 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | 1.2M ammonium sulfate, 0.75M Tris hydrochloride, 0.25M calcium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
