3GK8
X-ray crystal structure of the Fab from MAb 14, mouse antibody against Canine Parvovirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Collection date | 2005-05-02 |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 168.604, 39.885, 70.745 |
Unit cell angles | 90.00, 94.58, 90.00 |
Refinement procedure
Resolution | 42.020 - 2.000 |
Rwork | 0.266 |
R-free | 0.30600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.390 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
Rmerge | 0.061 | 0.021 | 0.902 |
Number of reflections | 40514 | ||
<I/σ(I)> | 25.819 | ||
Completeness [%] | 99.8 | 98.3 | 100 |
Redundancy | 3.6 | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25% PEG 5000, 0.1M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 298K |