3GK6
Crystal structure from the mobile metagenome of Vibrio cholerae. Integron cassette protein VCH_CASS2.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97953 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.378, 59.378, 95.756 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.041 - 1.800 |
R-factor | 0.1852 |
Rwork | 0.183 |
R-free | 0.22690 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.890 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.062 | 0.561 |
Number of reflections | 34832 | |
<I/σ(I)> | 21 | 3.6 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 9.9 | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 294 | 0.1M Citric acid, 25% PEG 3350, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |