3GJZ
Crystal structure of microcin immunity protein MccF from Bacillus anthracis str. Ames
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-10 |
Detector | ADSC QUANTUM Q315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 |
Unit cell lengths | 118.962, 118.962, 55.542 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.100 |
R-factor | 0.18893 |
Rwork | 0.187 |
R-free | 0.23176 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.689 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.093 | 0.561 |
Number of reflections | 45439 | |
<I/σ(I)> | 15.2 | 2.13 |
Completeness [%] | 99.3 | 97.8 |
Redundancy | 3.9 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 291 | 0.056M Sodium dihydrogen phosphate, 1.344M di-Potassium hydrogen phosphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |