3GJP
Crystal structure of mutant coiled coil GCN4 leucine zipper
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-09-27 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.246, 45.936, 47.156 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.900 - 2.000 |
R-factor | 0.215 |
Rwork | 0.212 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o7h |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.150 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 6442 | |
<I/σ(I)> | 15.7 | 7.6 |
Completeness [%] | 98.1 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |