3GIX
Crystal structure of human splicing factor dim2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2005-12-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.325, 63.640, 51.148 |
Unit cell angles | 90.00, 92.29, 90.00 |
Refinement procedure
Resolution | 30.570 - 1.330 |
R-factor | 0.14672 |
Rwork | 0.145 |
R-free | 0.19651 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xbs |
RMSD bond length | 0.027 |
RMSD bond angle | 2.243 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.570 | 1.380 |
High resolution limit [Å] | 1.330 | 1.330 |
Number of reflections | 204363 | |
<I/σ(I)> | 20.88 | 4.42 |
Completeness [%] | 97.0 | 89.6 |
Redundancy | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 18% PEG 8000, 0.2 M Magnesium acetate tetrahydate, 0.1 M Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |