3GIR
Crystal structure of glycine cleavage system aminomethyltransferase T from Bartonella henselae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-27 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.980, 72.670, 138.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.010 - 1.600 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.20500 |
| Structure solution method | MR, MR |
| Starting model (for MR) | 1wsr modified with ccp4 program chainsaw |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.845 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.010 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.044 | 0.493 |
| Number of reflections | 70368 | |
| <I/σ(I)> | 16.45 | 2.6 |
| Completeness [%] | 95.3 | 98.9 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | PROPLEX SCREEN, H4: 1.4M NA MALONATE, BAHEA.00657.A AT 44.8 MG/ML, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
