3GIE
Crystal structure of DesKC_H188E in complex with AMP-PCP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-09-17 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.890, 94.050, 44.620 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 27.165 - 2.650 |
R-factor | 0.199 |
Rwork | 0.194 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3EHG and 3EHF |
RMSD bond length | 0.008 |
RMSD bond angle | 1.440 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (2009_02_15_2320_3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.165 | 2.750 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.061 | |
Number of reflections | 11757 | |
Completeness [%] | 97.6 | 95.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | MPD, MgCl2, Hepes, AMP-PCP, pH 6, vapor diffusion, hanging drop, temperature 291K |