3GIB
Crystal Structure of the Complex of the E. coli Hfq with Poly(A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.534, 88.960, 39.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.500 - 2.400 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.223 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 89.087 | 89.090 | 2.530 |
| High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
| Rmerge | 0.051 | 0.034 | 0.446 |
| Total number of observations | 2180 | 9807 | |
| Number of reflections | 9714 | ||
| <I/σ(I)> | 8.142 | 15.3 | 1.7 |
| Completeness [%] | 99.9 | 99.1 | 99.8 |
| Redundancy | 6.8 | 6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 298 | 40% MPD, 0.1 M CHES 9.5, hanging drop, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | CHES | ||
| 3 | 1 | 2 | MPD | ||
| 4 | 1 | 2 | CHES |
