3GIA
Crystal Structure of ApcT Transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 113.482, 99.499, 72.693 |
Unit cell angles | 90.00, 117.71, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.320 |
R-factor | 0.20405 |
Rwork | 0.203 |
R-free | 0.23274 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gi9 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.049 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.320 | 2.320 |
Rmerge | 0.106 | 0.715 |
Number of reflections | 30472 | |
<I/σ(I)> | 18.6 | 2.1 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 7.4 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 9 | 293 | 0.1M Bicine, 0.1M sodium chloride, 39-45% PEG 555 MME, pH 9.0, Microbatch, temperature 293K |