3GI9
Crystal Structure of ApcT Transporter Bound to 7F11 Monoclonal Fab Fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.365, 45.900, 158.088 |
| Unit cell angles | 90.00, 97.75, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.480 |
| R-factor | 0.25033 |
| Rwork | 0.248 |
| R-free | 0.29568 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.937 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.052 | 0.302 |
| Number of reflections | 36003 | |
| <I/σ(I)> | 24.3 | 2.1 |
| Completeness [%] | 93.5 | 70.3 |
| Redundancy | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 293 | 0.1M sodium acetate, 0.1M rubidium chloride, 0.1M L-alanine, 40-48% PEG 400, pH 5.0, Microbatch, temperature 293K |
