3GI8
Crystal Structure of ApcT K158A Transporter Bound to 7F11 Monoclonal Fab Fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.039, 111.188, 115.674 |
| Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.590 |
| R-factor | 0.25629 |
| Rwork | 0.254 |
| R-free | 0.30685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ApcT - Fab complex |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.935 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.087 | 0.335 |
| Number of reflections | 36142 | |
| <I/σ(I)> | 17.4 | 3 |
| Completeness [%] | 99.6 | 96.8 |
| Redundancy | 4.4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 293 | 0.1M sodium acetate, 0.1M ammonium dihydrogen phosphate, 40-44% PEG 400, pH 5.0, Microbatch, temperature 293K |
