3GHQ
Crystal Structure of E. coli W35F BFR mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Wavelength(s) | 1.7429 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 207.641, 207.641, 142.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.610 - 2.700 |
R-factor | 0.24419 |
Rwork | 0.243 |
R-free | 0.26169 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3e1j |
RMSD bond length | 0.016 |
RMSD bond angle | 1.554 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 81605 | |
<I/σ(I)> | 17.2 | 3.8 |
Completeness [%] | 95.6 | 76.8 |
Redundancy | 10.4 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 289 | 2.45M Ammonium Sulfate, 0.1M sodium Citrate pH 5.0, temperature 289K |